Interference in immunoassay measurements of total and free phenytoin in uremic patients: a reappraisal.

@article{Roberts1993InterferenceII,
  title={Interference in immunoassay measurements of total and free phenytoin in uremic patients: a reappraisal.},
  author={William L. Roberts and Petrie M. Rainey},
  journal={Clinical chemistry},
  year={1993},
  volume={39 9},
  pages={1872-7}
}
Accumulation of phenytoin metabolites in uremia has been shown to interfere with some immunoassay methods for phenytoin measurement. We evaluated the effects of uremia (serum creatinine > 13 mg/L) on free and total phenytoin concentrations measured by the Abbott TDx fluorescence polarization immunoassay, and the DuPont aca and Syva EMIT homogeneous enzyme-multiplied immunoassay methods, using HPLC as the comparison method. In uremic patients, the TDx assay showed both fixed and proportional… CONTINUE READING