Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system

@inproceedings{Staals2016InterferencedrivenSA,
  title={Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system},
  author={Raymond H J Staals and Simon A Jackson and Ambarish Biswas and Stan J J Brouns and Chris M. Brown and Peter C. Fineran},
  booktitle={Nature communications},
  year={2016}
}
CRISPR-Cas systems provide bacteria with adaptive immunity against foreign nucleic acids by acquiring short, invader-derived sequences called spacers. Here, we use high-throughput sequencing to analyse millions of spacer acquisition events in wild-type populations of Pectobacterium atrosepticum. Plasmids not previously encountered, or plasmids that had escaped CRISPR-Cas targeting via point mutation, are used to provoke naive or primed spacer acquisition, respectively. The origin, location and… CONTINUE READING

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