Interference by naturally occurring fatty acids in a noncellular enzyme-based aromatase bioassay.

@article{Balunas2006InterferenceBN,
  title={Interference by naturally occurring fatty acids in a noncellular enzyme-based aromatase bioassay.},
  author={Marcy J. Balunas and Bin Su and Serena Landini and Robert W. Brueggemeier and A Douglas Kinghorn},
  journal={Journal of natural products},
  year={2006},
  volume={69 4},
  pages={700-3}
}
Natural product drug discovery efforts frequently utilize noncellular screening assays. Fatty acids are commonly found in natural product extracts, and some have been shown to interfere with noncellular assays. Several pure fatty acids were tested using a noncellular aromatase assay, with the unsaturated analogues showing strong inhibitory activity, while the saturated analogues were inactive. Unsaturated fatty acids were further tested against SK-BR-3 hormone-independent human breast cancer… CONTINUE READING