Interfacial activation-based molecular bioimprinting of lipolytic enzymes.

  title={Interfacial activation-based molecular bioimprinting of lipolytic enzymes.},
  author={Ismael Mingarro and Concepci{\'o}n Abad and L Braco},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={92 8},
Interfacial activation-based molecular (bio)-imprinting (IAMI) has been developed to rationally improve the performance of lipolytic enzymes in nonaqueous environments. The strategy combinedly exploits (i) the known dramatic enhancement of the protein conformational rigidity in a water-restricted milieu and (ii) the reported conformational changes associated with the activation of these enzymes at lipid-water interfaces, which basically involves an increased substrate accessibility to the… CONTINUE READING