Interface of an array of five capillaries with an array of one-nanoliter wells for high-resolution electrophoretic analysis as an approach to high-throughput chemical cytometry.

@article{Boardman2008InterfaceOA,
  title={Interface of an array of five capillaries with an array of one-nanoliter wells for high-resolution electrophoretic analysis as an approach to high-throughput chemical cytometry.},
  author={Anna K. Boardman and Sarah C. McQuaide and Cuiru Zhu and Colin D. Whitmore and M. Lidstrom and N. Dovichi},
  journal={Analytical chemistry},
  year={2008},
  volume={80 19},
  pages={
          7631-4
        }
}
We report a system that allows the simultaneous aspiration of one or more cells into each of five capillaries for electrophoresis analysis. A glass wafer was etched to create an array of 1-nL wells. The glass was treated with poly(2-hydroxyethyl methacrylate) to control cell adherence. A suspension of formalin-fixed cells was placed on the surface, and cells were allowed to settle. The concentration of cells and the settling time were chosen so that there was, on average, one cell per well… Expand
13 Citations
Analysis of intracellular reducing levels in human hepatocytes on three-dimensional focusing microchip.
  • Chunxiu Xu, Longfei Cai
  • Chemistry, Medicine
  • Luminescence : the journal of biological and chemical luminescence
  • 2014
Sampling techniques for single-cell electrophoresis.
  • 26
Chemical fixation to arrest phospholipid signaling for chemical cytometry.
  • 5
Chemical analysis of single cells.
  • 173
  • PDF
...
1
2
...