Interdomain signalling in the blue-light sensing and GTP-binding protein YtvA: a mutagenesis study uncovering the importance of specific protein sites.

@article{Tang2010InterdomainSI,
  title={Interdomain signalling in the blue-light sensing and GTP-binding protein YtvA: a mutagenesis study uncovering the importance of specific protein sites.},
  author={Yifen Tang and Zhen Yu Cao and Elsa Livoti and Ulrich Krauss and K. -E. J{\"a}ger and Wolfgang G{\"a}rtner and Aba Losi},
  journal={Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology},
  year={2010},
  volume={9 1},
  pages={47-56}
}
YtvA from Bacillus subtilis is a blue-light responsive, flavin-binding photoreceptor, built of a light-sensing LOV domain (aa 25-126) and an NTP (nucleoside triphosphate)-binding STAS domain (aa 147-261). The STAS domain is supposed to be the effector part of the protein or a secondary switch. Both domains are connected by a linker polypeptide. The active form of YtvA is generated upon light excitation, causing the formation of a covalent bond between a cysteine residue (Cys62) in the LOV… CONTINUE READING