Interconversion of metaphase and interphase microtubule arrays, as studied by the injection of centrosomes and nuclei into Xenopus eggs

@article{Karsenti1984InterconversionOM,
  title={Interconversion of metaphase and interphase microtubule arrays, as studied by the injection of centrosomes and nuclei into Xenopus eggs},
  author={{\'E}ric Karsenti and John F. Newport and R Hubble and Marc Kirschner},
  journal={The Journal of Cell Biology},
  year={1984},
  volume={98},
  pages={1730 - 1745}
}
We have designed experiments that distinguish centrosomal , nuclear, and cytoplasmic contributions to the assembly of the mitotic spindle. Mammalian centrosomes acting as microtubule-organizing centers were assayed by injection into Xenopus eggs either in a metaphase or an interphase state. Injection of partially purified centrosomes into interphase eggs induced the formation of extensive asters. Although centrosomes injected into unactivated eggs (metaphase) did not form asters, inhibition of… CONTINUE READING

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