Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation.

Abstract

Assembly of the active site of the [NiFe]-hydrogenase enzymes involves a multi-step pathway and the coordinated activity of many accessory proteins. To analyze complex formation between these factors in Escherichia coli, they were genomically tagged and native multi-protein complexes were isolated. This method validated multiple interactions reported in… (More)

Topics

Cite this paper

@article{Butland2006InteractionsOT, title={Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation.}, author={Gareth Butland and Jie Zhang and Wenhong Yang and Anthony Sheung and Peter Wong and Jack Greenblatt and Andrew Emili and Deborah B. Zamble}, journal={FEBS letters}, year={2006}, volume={580 2}, pages={677-81} }