Interactions of polymeric and liposomal gene delivery systems with extracellular glycosaminoglycans: physicochemical and transfection studies.

@article{Ruponen1999InteractionsOP,
  title={Interactions of polymeric and liposomal gene delivery systems with extracellular glycosaminoglycans: physicochemical and transfection studies.},
  author={Marika Ruponen and Seppo Yl{\"a}-Herttuala and Arto Urtti},
  journal={Biochimica et biophysica acta},
  year={1999},
  volume={1415 2},
  pages={331-41}
}
Complexes of DNA with cationic lipids and cationic polymers are frequently used for gene transfer. Extracellular interactions of the complexes with anionic glycosaminoglycans (GAGs) may interfere with gene transfer. Interactions of GAGs with the carrier-DNA complexes were studied using tests for DNA relaxation (ethidium bromide intercalation), DNA release (electrophoresis), and transfection (pCMVbetaGal transfer into RAA smooth muscle cells). Several cationic lipid formulations (DOTAP, DOTAP… CONTINUE READING

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