Interactions of calcium and native tropomyosin with myosin and heavy meromyosin.
@article{Kominz1966InteractionsOC,
title={Interactions of calcium and native tropomyosin with myosin and heavy meromyosin.},
author={D. R. Kominz},
journal={Archives of biochemistry and biophysics},
year={1966},
volume={115 3},
pages={
583-92
}
}Abstract It is confirmed that the presence of native tropomyosin is required in order for the removal of Ca ++ to cause inhibition of the Mg ++ -activated ATPase of actomyosin and acto-heavy-meromyosin. Native tropomyosin is present in routinely purified samples of myosin at levels of 0.7–2.3%; such quantities inhibit the actomyosin ATPase by 30–60% upon removal of Ca ++ . Titration with mercurial at low ionic strength causes a loss of actomyosin ATPase; it causes an even greater loss of the… CONTINUE READING