Interactions of USF and Ku antigen with a human DNA region containing a replication origin.

@article{Tth1993InteractionsOU,
  title={Interactions of USF and Ku antigen with a human DNA region containing a replication origin.},
  author={Ecaterina T{\'o}th and L Marusic and A. Ochem and Andreas Patthy and S{\'a}ndor Pongor and Mauro Giacca and A. Falaschi},
  journal={Nucleic acids research},
  year={1993},
  volume={21 14},
  pages={3257-63}
}
By means of a combination of ion-exchange and sequence-specific affinity chromatography techniques, we have purified to homogeneity two protein complexes binding in a human DNA region (B48) previously recognized to contain a DNA replication origin. The DNA sequence used for the protein purification (B48 binding site) contains a binding site for basic-helix-loop-helix DNA binding proteins. The first complex is composed of two polypeptides of 42- and 44-kDa; its size, heat stability, and target… CONTINUE READING
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Molecular Cloning— A laboratory manual/n Edition

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  • 1989
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