Interactions of Streptomyces subtilisin inhibitor with Streptomyces griseus proteases A and B. Enzyme kinetic and computer simulation studies.

@article{Christensen1985InteractionsOS,
  title={Interactions of Streptomyces subtilisin inhibitor with Streptomyces griseus proteases A and B. Enzyme kinetic and computer simulation studies.},
  author={Ulla Christensen and Shin-ichi Ishida and Seiji Ishii and Yukio Mitsui and Yoichi Iitaka and Judith A. McClarin and Robert Langridge},
  journal={Journal of biochemistry},
  year={1985},
  volume={98 5},
  pages={
          1263-74
        }
}
Streptomyces subtilisin inhibitor (SSI), a dimeric protein that strongly inhibits subtilisins, was shown to form tight inhibitory complexes with Streptomyces griseus proteases A and B (SGPA and SGPB). The apparent dissociation constants of the SGPA-SSI and SGPB-SSI complexes were found to be orders of magnitude less than those of subtilisin-SSI complexes. Using the known atomic coordinates for SGPA and SSI, the highly complementary nature of the surface geometries of the two proteins was… CONTINUE READING
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