Interactions between protein-gold complexes and cell surfaces: a method for precise quantitation.

@article{Kehle1987InteractionsBP,
  title={Interactions between protein-gold complexes and cell surfaces: a method for precise quantitation.},
  author={T Kehle and Volker Herzog},
  journal={European journal of cell biology},
  year={1987},
  volume={45 1},
  pages={
          80-7
        }
}
We have developed a rapid and precise electron microscope technique for the quantitation of gold particles in suspension using latex microspheres as a reference (EM latex technique). This technique allowed us to determine the specific absorption of colloidal gold at its absorption maximum (520 nm) and the average number of ligands ([125I]IgG) bound to one gold particle. On the basis of these values important binding characteristics of protein-gold complexes to cell surfaces were analyzed in a… CONTINUE READING