Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin T.

Abstract

The transcriptional transactivator Tat from HIV binds to the transactivation response element (TAR) RNA to increase rates of elongation of viral transcription. Human cyclin T supports these interactions between Tat and TAR. In this study, we report the sequence of mouse cyclin T and identify the residues from positions 1 to 281 in human cyclin T that bind to Tat and TAR. Mouse cyclin T binds to Tat weakly and is unable to facilitate interactions between Tat and TAR. Reciprocal exchanges of the cysteine and tyrosine at position 261 in human and mouse cyclin T proteins also render human cyclin T inactive and mouse cyclin T active. These findings reveal the molecular basis for the restriction of Tat transactivation in rodent cells.

5 Figures and Tables

0102030'01'03'05'07'09'11'13'15'17
Citations per Year

134 Citations

Semantic Scholar estimates that this publication has 134 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Fujinaga1999InteractionsBH, title={Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin T.}, author={Koh Fujinaga and Ran Taube and Johannes Wimmer and Thomas P. Cujec and B. Matija Peterlin}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1999}, volume={96 4}, pages={1285-90} }