Interaction sites of tropomyosin in muscle thin filament as identified by site-directed spin-labeling.

@article{Ueda2011InteractionSO,
  title={Interaction sites of tropomyosin in muscle thin filament as identified by site-directed spin-labeling.},
  author={Keisuke Ueda and Chieko Kimura-Sakiyama and Tomoki Aihara and Masao Miki and Toshiaki Arata},
  journal={Biophysical journal},
  year={2011},
  volume={100 10},
  pages={2432-9}
}
To identify interaction sites we measured the rotational motion of a spin label covalently bound to the side chain of a cysteine genetically incorporated into rabbit skeletal muscle tropomyosin (Tm) at positions 13, 36, 146, 160, 174, 190, 209, 230, 271, and 279. Upon the addition of F-actin, the mobility of all the spin labels, especially at position 13… CONTINUE READING