Interaction of zonula occludens-1 (ZO-1) with alpha-actinin-4: application of functional proteomics for identification of PDZ domain-associated proteins.

@article{Chen2006InteractionOZ,
  title={Interaction of zonula occludens-1 (ZO-1) with alpha-actinin-4: application of functional proteomics for identification of PDZ domain-associated proteins.},
  author={Vincent C W Chen and Xinbo Li and H{\'e}l{\`e}ne Perreault and J. I. Nagy},
  journal={Journal of proteome research},
  year={2006},
  volume={5 9},
  pages={2123-34}
}
The use of recombinant "bait" proteins to capture protein-binding partners, followed by identification of protein interaction networks by mass spectrometry (MS), has gained popularity and widespread acceptance. We have developed an approach using recombinant PDZ protein interaction modules of the membrane-associated guanylate kinase (MAGUK) protein zonula occludens-1 (ZO-1) to pull-down and screen for proteins that interact with these modules via their PDZ domain binding motifs. Identification… CONTINUE READING