Interaction of the hydrogenase accessory protein HypC with HycE, the large subunit of Escherichia coli hydrogenase 3 during enzyme maturation.

@article{Drapal1998InteractionOT,
  title={Interaction of the hydrogenase accessory protein HypC with HycE, the large subunit of Escherichia coli hydrogenase 3 during enzyme maturation.},
  author={Nikola M Drapal and August Bock},
  journal={Biochemistry},
  year={1998},
  volume={37 9},
  pages={2941-8}
}
Maturation of the large subunit of E. coli hydrogenase 3, HycE, requires the action of seven accessory proteins. The HycI protease catalyses a C-terminal proteolytic cleavage of the large subunit, which was shown to result in a dramatic change in migration behavior of HycE in nondenaturing PAGE. HypA, HypB, HypC, HypD, HypE, and HypF are required for metallocenter assembly. A polyacrylamide gel system under nondenaturing conditions was used for the investigation of any protein-protein… CONTINUE READING