Interaction of the cyclophosphamide metabolite, acrolein, with lactate dehydrogenase-m homolog.

@article{Scappaticci1993InteractionOT,
  title={Interaction of the cyclophosphamide metabolite, acrolein, with lactate dehydrogenase-m homolog.},
  author={F Scappaticci and A. E. Maccubbin and N S Srikanth and Michael B. Faletto and Hira L. Gurtoo},
  journal={International journal of oncology},
  year={1993},
  volume={2 4},
  pages={
          683-93
        }
}
The cyclophosphamide metabolite, acrolein, binds to both nucleic acids and proteins. In this report, acrolein is shown to interact preferentially with a rat liver 35KD DNA-binding protein. The 35KD protein was purified to homogeneity from a hepatic microsomal fraction using (NH4)2SO4 precipitation, DEAE-Sepharose chromatography, cation exchange HPLC, and DNA-Sepharose affinity chromatography. The protein was identified as a homologue of lactate dehydrogenase (LDH) M subunit on the basis of… CONTINUE READING