Interaction of the adeno-associated virus Rep protein with a sequence within the A palindrome of the viral terminal repeat.

@article{Mccarty1994InteractionOT,
  title={Interaction of the adeno-associated virus Rep protein with a sequence within the A palindrome of the viral terminal repeat.},
  author={Doug Mccarty and Josh Ryan and Sergei Zolotukhin and Xiulan Zhou and Nicholas Muzyczka},
  journal={Journal of virology},
  year={1994},
  volume={68 8},
  pages={4998-5006}
}
We have characterized a Rep binding sequence which is within the A stem region of the adeno-associated virus terminal repeat (TR) and compared its affinity with that of the complete hairpinned TR for pure Rep68. Both the A stem and the complete TR substrates produced a complex pattern of protein-DNA complexes in which at least six different bound species could be distinguished. Competition experiments suggested that the dissociation constant for the A stem sequence is approximately 125-fold… CONTINUE READING

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