Interaction of the IciA protein with AT-rich regions in plasmid replication origins.

@article{Wei1996InteractionOT,
  title={Interaction of the IciA protein with AT-rich regions in plasmid replication origins.},
  author={T Wei and Rolf Bernander},
  journal={Nucleic acids research},
  year={1996},
  volume={24 10},
  pages={1865-72}
}
A set of AT-rich repeats is a common motif in prokaryotic replication origins. We have screened for proteins binding to the AT-rich repeat region in plasmids F, R1 and pSC101 using an electrophoretic mobility shift assay with PCR-amplified DNA fragments from the origins. The IciA protein, which is known to bind to the AT-rich repeat region in the Escherichia coli origin of chromosome replication, oriC, was found to bind to the corresponding region from plasmids F (oriS) and R1, but not to… CONTINUE READING

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