Interaction of synthetic peptide octarphin with human blood lymphocytes

  title={Interaction of synthetic peptide octarphin with human blood lymphocytes},
  author={Yu. N. Nekrasova and Yu. A. Zolotarev and Elena V. Navolotskaya},
  journal={Biochemistry (Moscow)},
The synthetic peptide octarphin (TPLVTLFK, fragment 12–19 of β-endorphin), a selective agonist of nonopioid β-endorphin receptor, was prepared with specific activity 28 Ci/mmol. The binding of [3H]octarphin to T and B lymphocytes isolated from the blood of donors was studied. It was found that [3H]octarphin binds both to T and B cells with high affinity: Kd = 3.0 ± 0.2 and 3.2 ± 0.3 nM, respectively. The specific binding of [3H]octarphin to T and B lymphocytes was competitively inhibited by… 
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