Interaction of sphingomyelins and phosphatidylcholines with fluorescent dehydroergosterol.

@article{Schroeder1989InteractionOS,
  title={Interaction of sphingomyelins and phosphatidylcholines with fluorescent dehydroergosterol.},
  author={Friedhelm Schroeder and Gyorgy Nemecz},
  journal={Biochemistry},
  year={1989},
  volume={28 14},
  pages={
          5992-6000
        }
}
The fluorescent sterol dehydroergosterol was used as a cholesterol analogue in conjunction with multifrequency phase and modulation (1-250 MHz) fluorometry to examine whether sterols (1) interact preferentially with fluid- or solid-phase phospholipids and (2) interact preferentially with sphingomyelin in phase-separated or phase-miscible cosonicated phospholipid membranes. Cosonicated small unilamellar vesicles (SUV) were produced by mixing lipids in organic solvents, drying the mixture, adding… CONTINUE READING

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