Interaction of soybean beta-amylase with glucose.

@article{Nomura1986InteractionOS,
  title={Interaction of soybean beta-amylase with glucose.},
  author={Kohji Nomura and Bunzo Mikami and Yasutaka Morita},
  journal={Journal of biochemistry},
  year={1986},
  volume={100 5},
  pages={
          1175-83
        }
}
The interaction of soybean beta-amylase with glucose was investigated by inhibition kinetics studies and spectroscopic measurements. The inhibition type, inhibitor constant (Ki) and dissociation constant (Kd) of beta-amylase-glucose complex were dependent on pH. At pH 8.0, glucose behaved as a competitive inhibitor (Ki = 34 mM). Binding of glucose produced a characteristic difference spectrum and a change of circular dichroism (CD) at pH 8.1. By using difference absorbance at 292 nm and… CONTINUE READING
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