Interaction of soluble and surface-bound heparin binding growth-associated molecule with heparin.

@article{Fath1999InteractionOS,
  title={Interaction of soluble and surface-bound heparin binding growth-associated molecule with heparin.},
  author={Melissa A. Fath and Victoria A Vandernoot and Ilkka Kilpel{\"a}inen and Tarja Inkeri Kinnunen and Heikki Rauvala and Robert J. Linhardt},
  journal={FEBS letters},
  year={1999},
  volume={454 1-2},
  pages={105-8}
}
The interaction of heparin with heparin binding growth-associated molecule (HB-GAM) was studied using isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR). ITC studies showed that, in solution, heparin bound HB-GAM with a deltaH of -30 kcal/mole corresponding to a dissociation constant (Kd) of 460 nM. The stoichiometry of interaction… CONTINUE READING