Interaction of pyridostigmine and physical stress on antioxidant defense system in skeletal muscle of mice

  title={Interaction of pyridostigmine and physical stress on antioxidant defense system in skeletal muscle of mice},
  author={Ramesh Jagannathan and Kazim Husain and Satu M. Somani},
  journal={Journal of Applied Toxicology},
Pyridostigmine bromide (PB), a reversible anticholinesterase drug, had been used against possible nerve gas exposure during the Persian Gulf War. The Gulf War veterans used PB and they were under physical stress. This study investigated the delayed and interactive effects of pyridostigmine and physical stress on the antioxidant defense system in triceps muscle of mice. Male NIH Swiss mice were divided into four groups and treated as follows: sedentary control; pyridostigmine (1.2 mg kg−1 p.o… Expand
Persistent/delayed toxic effects of low-dose sarin and pyridostigmine under physical stress (exercise) in mice.
It is concluded that physical stress (exercise) enhanced the persistent/delayed toxic effects of low-dose sarin and pyridostigmine in specific tissues of mice. Expand
Cholinesterase inhibitors and stress: effects on brain muscarinic receptor density in mice.
The hypothesis that PB exposure coupled with stress will alter cholinergic receptor density is explored based on the rationale that prolonged exposure to PB and stress will lead to increased stimulation of cholinesterase receptors due to the reduced capacity to degrade acetylcholine, leading to changes in receptor levels. Expand
Vulnerability of the mid aged rat myocardium to the age-induced oxidative stress: Influence of exercise training on antioxidant defense system
The findings of the present study suggest that, although the activity levels of the myocardial antioxidant enzymes were elevated with the 12 weeks of exercise training, the changes were not sufficient enough in attenuating oxidative stress in theMyocardium of female rats during this short period of exerciseTraining. Expand
Oxidative modifications of blood serum proteins in myasthenia gravis
Results demonstrate systemic oxidative stress in MG, suggesting therapeutic use of antioxidants and FRAP and ABTS• scavenging by fast antioxidants were unchanged, but ABTS • scavengingBy slow antioxidants was lower in OMGb and GMGc, in patients ill for >5cy, in Patients with lowc and highb Ab (ap<0.05, bp <0.01, cp.<0.001). Expand
Effects of Intermittent Fasting and Physical Activity on Salivary Expression of Reduced Glutathione and Interleukin-1β.
Results suggest that a combination of moderate physical activity and intermittent fasting promotes the maintenance of antioxidant function while inhibiting the inflammatory process. Expand
Do vasoactive neuropeptide autoimmune disorders explain pyridostigmine's association with Gulf War syndrome?
A possible link between exposures to PB and other chemical, physical and psychological stressors in producing a fatigue-related illness possibly related to autoimmune dysfunction of certain VNs is explored. Expand
Lysine-specific demethylase 1 inhibitors protect cochlear spiral ganglion neurons against cisplatin-induced damage
Findings show that LSD1 inhibitors prevent cisplatin-induced SGN loss by regulating the demethylation of H3K4 and preventing increases of reactive oxygen species levels, which might provide a potential therapeutic strategy for cisplasin-induced hearing loss. Expand
Gulf War related exposure factors influencing topical absorption of 14C-permethrin.
It is suggested that co-exposure to JP-8 or sulfur mustard may modulate transdermal flux of 14C-permethrin and this moderate increase in permethrin absorption remains unclear. Expand
Percutaneous Absorption of Topical N , N -Diethyl- m -Toluamide (Deet): Effects of Exposure Variables and Coadministered Toxicants
The results suggest that coexposure to a number of chemicals that potentially could be encountered in a military environment may modulate the percutaneous absorption of topically applied DEET beyond that seen for normal vehicles at typically applied concentrations. Expand


Interactive and delayed effects of pyridostigmine and physical stress on biochemical and histological changes in peripheral tissues of mice
Investigation of the delayed effects of pyridostigmine and treadmill exercise on cholinesterase activity, lipid peroxidation and histology of peripheral tissues of mice indicates that physical stress enhanced the delayed toxic effects of a subchronic oral dose of pyrsidostIGmine primarily in the skeletal muscle of mice. Expand
Study of muscular effects of short-term pyridostigmine treatment in resting and exercising rats
No evidence was found of a deleterious effect of this treatment of Pyridostigmine on the skeletal muscle, and following physical exercise, the same treat ment significantly exacerbated the biochemical changes reflecting a loss of integrity in skeletal muscles, namely, increased CPK and urinary creatine excretion rate. Expand
Response of cardiac antioxidant system to alcohol and exercise training in the rat.
The data suggest that ET may reduce the extent of the damage caused by ethanol consumption on the myocardium, and augments the antioxidant enzyme activity and GSH levels in the heart. Expand
Responses of antioxidant system to acute and trained exercise in rat heart subcellular fractions
Acute exercise resulted in a larger increase in enzyme activities than trained exercise, possibly as a compensatory mechanism to cope with the enhanced production of superoxides and oxyradicals during exhaustive exercise. Expand
Effect of repeated treatment with pyridostigmine on acetylcholinesterase in mouse muscles
Repeated treatment with pyridostigmine caused de layed changes in functional acetylcholinesterase in diaphragm, and altered the sensitivity of the muscles to the drug. Expand
Effects of exposure to low‐dose pyridostigmine on neuromuscular junctions in vitro
The reversibility of the PB‐induced changes in vitro suggests that such changes are causally unrelated to the fatigue reported by Persian Gulf War veterans years after exposure to PB. Expand
Ultrastructural effects of pyridostigmine on neuromuscular junctions in rat diaphragm.
Pyridostigmine bromide, a quaternary carbamate, is widely used in treatment of myasthenia gravis and has been suggested for use in prophylaxis against intoxication with irreversible cholinesteraseExpand
Differential regulation of manganese superoxide dismutase activity by alcohol and TNF in human hepatoma cells.
Findings are compatible with the view that chronic exposure to ethanol suppresses the cellular adaptive response to oxidative stress, and if this adaptive response of MnSOD is lessened, it may have implications in the increased toxicity due to prolonged ethanol exposure. Expand
Influence of exercise and ethanol on cholinesterase activity and lipid peroxidation in blood and brain regions of rat
  • K. Husain, S. Somani
  • Medicine, Chemistry
  • Progress in Neuro-Psychopharmacology and Biological Psychiatry
  • 1997
The interaction of acute exercise and single ethanol intake on cholinergic enzyme and its relationship to lipid peroxidation in the blood and brain regions of the rat was elucidates and inhibition of striatal AChE was correlated with increased lipidperoxidation indicating perturbation of motor function. Expand
Neuromuscular toxicity of pyridostigmine bromide in the diaphragm, extensor digitorum longus, and soleus muscles of the rat.
The data indicate that acute or subacute exposure to pyridostigmine bromide at a whole blood ChE depression of 60-70% results in similar alterations to the NMJs of three muscles with substantially different fiber type compositions. Expand