Interaction of nucleolar protein B23 with peptides related to nuclear localization signals.

@article{Szebeni1995InteractionON,
  title={Interaction of nucleolar protein B23 with peptides related to nuclear localization signals.},
  author={Attila Szebeni and Jorge E Herrera and Mark O. J. Olson},
  journal={Biochemistry},
  year={1995},
  volume={34 25},
  pages={8037-42}
}
Nucleolar protein B23 is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The interactions of various NLS-containing peptides with two B23 isoforms (B23.1 and B23.2) were examined using equilibrium dialysis and Scatchard analyses. The KD for protein B23 binding to a peptide containing the SV40 T-antigen NLS sequence was approximately 1 microM with a stoichiometry of 1:1 (peptide:protein). No significant differences were seen… CONTINUE READING

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