Interaction of nerve growth factor with surface membranes: biological competence of insolubilized nerve growth factor.

@article{Frazier1973InteractionON,
  title={Interaction of nerve growth factor with surface membranes: biological competence of insolubilized nerve growth factor.},
  author={William Allen Frazier and Lisa F Boyd and Ralph A Bradshaw},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1973},
  volume={70 10},
  pages={
          2931-5
        }
}
Nerve growth factor was insolubilized by covalent attachment to Sepharose beads. Nerve growth factor-Sepharose was biologically active in both the neurite outgrowth assay for nerve growth factor and in preserving responsive neurons in vitro. Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine… CONTINUE READING

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