Interaction of lactoferrin with ceruloplasmin.

  title={Interaction of lactoferrin with ceruloplasmin.},
  author={Elena T. Zakharova and Mikhail M. Shavlovski and Mikhail G Bass and Anastasia A Gridasova and Maria O. Pulina and Vincenzo De Filippis and Mariano Beltramini and Paolo Di Muro and Benedetto Salvato and Angelo Fontana and Vadim B. Vasilyev and V. Gaitskhoki},
  journal={Archives of biochemistry and biophysics},
  volume={374 2},
When added to human blood serum, the iron-binding protein lactoferrin (LF) purified from breast milk interacts with ceruloplasmin (CP), a copper-containing oxidase. Selective binding of LF to CP is evidenced by the results of polyacrylamide gel electrophoresis, immunodiffusion, gel filtration, and affinity chromatography. The molar stoichiometry of CP:LF in the complex is 1:2. Near-uv circular dichroism spectra of the complex showed that neither of the two proteins undergoes major structural… CONTINUE READING


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