Interaction of ketamine with μ2 opioid receptors in SH-SY5Y human neuroblastoma cells

  title={Interaction of ketamine with $\mu$2 opioid receptors in SH-SY5Y human neuroblastoma cells},
  author={Kazuyoshi Hirota and Kulvinder S. Sikand and David G Lambert},
  journal={Journal of Anesthesia},
AbstractPurpose. Ketamine is known to interact with opioid receptors. However, because this agent does not produce opioid-like respiratory depression, it might not interact with μ2 opioid receptors. Therefore, we have studied the interaction of ketamine with μ2 opioid receptors expressed in SH-SY5Y cells. Methods. SH-SY5Y cells (passage 70–80) were used to obtain ketamine dose-response curves for inhibition of 0.4 nM [3H][d-Ala2,MePhe4,Gly(ol)5] enkephalin (DAMGO) binding to μ2 opioid receptors… 
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