Corpus ID: 20334472

Interaction of dolastatin 10 with tubulin: induction of aggregation and binding and dissociation reactions.

@article{Bai1995InteractionOD,
  title={Interaction of dolastatin 10 with tubulin: induction of aggregation and binding and dissociation reactions.},
  author={R. Bai and G. F. Taylor and J. Schmidt and M. Williams and J. Kepler and G. Pettit and E. Hamel},
  journal={Molecular pharmacology},
  year={1995},
  volume={47 5},
  pages={
          965-76
        }
}
We have prepared [3H]dolastatin 10 and examined its interactions with tubulin. Binding kinetics appeared to be biphasic, with a rapid initial reaction that could not be accurately measured, followed by a slower second reaction. Bound drug was stable in centrifugal gel filtration, column gel filtration, and high performance liquid chromatography gel filtration, but the bound drug could be displaced by an active isomer of dolastatin 10. Scatchard analysis of binding data was consistent with two… Expand
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Sustained intracellular retention of dolastatin 10 causes its potent antimitotic activity.
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