Interaction of cytochrome c with reaction centers of Rhodopseudomonas sphaeroides R-26: determination of number of binding sites and dissociation constants by equilibrium dialysis.

@article{Rosen1980InteractionOC,
  title={Interaction of cytochrome c with reaction centers of Rhodopseudomonas sphaeroides R-26: determination of number of binding sites and dissociation constants by equilibrium dialysis.},
  author={Daniel Rosen and Melvin Y Okamura and George Feher},
  journal={Biochemistry},
  year={1980},
  volume={19 25},
  pages={5687-92}
}
The number of binding sites and dissociation constants of cytochrome c (horse heart) and cytochrome c2 (Rhodopseudomonas sphaeroides) to reaction centers of Rhodopseudomonas sphaeroides R-26 was determined by equilibrium dialysis. One binding site was found for both cytochromes. The dissociation constants (10 mM Tris-HCl, pH 8.0) were approximately 0.4 microM and approximately 1.0 microM for cytochrome c and cytochrome C2 respectively. Oxidized and reduced forms of both cytochromes bound to… CONTINUE READING