Interaction of cultured mammalian cells with WR-2721 and its thiol, WR-1065: implications for mechanisms of radioprotection.

@article{Purdie1983InteractionOC,
  title={Interaction of cultured mammalian cells with WR-2721 and its thiol, WR-1065: implications for mechanisms of radioprotection.},
  author={J W Purdie and E R Inhaber and H. Schneider and J L Labelle},
  journal={International journal of radiation biology and related studies in physics, chemistry, and medicine},
  year={1983},
  volume={43 5},
  pages={517-27}
}
An isothermal microcalorimeter was used to measure changes in heat flow when radioprotective drugs were added to cultured mammalian cells. The heat produced when WR-2721 was added continued for at least 90 min. WR-2721 was dephosphorylated by the cells to thiol (WR-1065) which oxidizes to disulphide. In the microcalorimeter, thiols give an immediate burst of heat due to this oxidation. A biological oxygen monitor revealed that WR-1065 and cysteamine rapidly consumed all the oxygen in culture… CONTINUE READING

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