Interaction of bovine rhodopsin with calcium ions

@article{Nll2004InteractionOB,
  title={Interaction of bovine rhodopsin with calcium ions},
  author={Gilbert N{\"o}ll and Hennig Stieve},
  journal={Biophysics of structure and mechanism},
  year={2004},
  volume={5},
  pages={43-53}
}
The formation of metarhodopsin II in various bovine rhodopsin preparations (rod outer segment (ROS) suspensions and rhodopsin-detergent solutions) was measured by means of flash spectrophotometry. The half-lifetime and formation of metarhodopsin II in ROS did not depend on the calcium concentration in the range of less than 10−9 M (using EGTA or EDTA) to 15×10−3 M calcium at pH values of 5.0, 7.1, and 9.0 (Table 1). The regeneration of rhodopsin from opsin by adding 11-cis retinal to ROS… CONTINUE READING