Interaction of bHLH-PAS proteins involved in juvenile hormone reception in Drosophila.

Abstract

The Methoprene-tolerant (Met) bHLH-PAS gene is involved in juvenile hormone (JH) action in Drosophila melanogaster as a likely component of a JH receptor. We expressed Met in Drosophila S2 cells and explored for MET partners using pull-down assays. MET-MET interaction was found to occur. The germ-cell expressed (gce) gene is another D. melanogaster bHLH-PAS gene with high homology to Met, and GCE formed heterodimers with MET. In the presence of JH or either of two JH agonists, MET-MET and MET-GCE formation was drastically reduced. Interaction between GCE and MET having N- or C-terminus truncations, bHLH or PAS-A domain deletions, or a point mutation in the PAS-B domain failed to occur. However, JH-dependent interaction occurred between GCE and MET having point mutations in bHLH or PAS-A. During development, changes in JH titer may alter partner binding by MET and result in different gene expression patterns.

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@article{Godlewski2006InteractionOB, title={Interaction of bHLH-PAS proteins involved in juvenile hormone reception in Drosophila.}, author={Jakub Godlewski and Shaoli Wang and Thomas G. Wilson}, journal={Biochemical and biophysical research communications}, year={2006}, volume={342 4}, pages={1305-11} }