Interaction of assembly protein AP-2 and its isolated subunits with clathrin.

  title={Interaction of assembly protein AP-2 and its isolated subunits with clathrin.},
  author={Kowdle Prasad and James H. Keen},
  volume={30 22},
The clathrin assembly protein complex AP-2 is a multimeric subunit complex consisting of two 100-115-kDa subunits known as alpha and beta and 50- and 16-kDa subunits. The subunits have been dissociated and separated by ion-exchange chromatography in 7.5 M urea. Fractions highly enriched in either the alpha or beta subunit were obtained. The alpha fraction interacted with clathrin as evidenced by its ability to bind to preassembled clathrin cages. It also reacted with dissociated clathrin… CONTINUE READING
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