Interaction of alpha-phenyl-N-tert-butyl nitrone and alternative electron acceptors with complex I indicates a substrate reduction site upstream from the rotenone binding site.

@article{Hensley1998InteractionOA,
  title={Interaction of alpha-phenyl-N-tert-butyl nitrone and alternative electron acceptors with complex I indicates a substrate reduction site upstream from the rotenone binding site.},
  author={Kenneth Hensley and Quentin N. Pye and M L Maidt and Charles A. Stewart and Katherine A. Robinson and F Jaffrey and Robert A. Floyd},
  journal={Journal of neurochemistry},
  year={1998},
  volume={71 6},
  pages={2549-57}
}
Mitochondrial complexes I, II, and III were studied in isolated brain mitochondrial preparations with the goal of determining their relative abilities to reduce O2 to hydrogen peroxide (H2O2) or to reduce the alternative electron acceptors nitroblue tetrazolium (NBT) and diphenyliodonium (DPI). Complex I and II stimulation caused H2O2 formation and reduced NBT and DPI as indicated by dichlorodihydrofluorescein oxidation, nitroformazan precipitation, and DPI-mediated enzyme inactivation. The O2… CONTINUE READING
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