Interaction of a hydroxyproline-rich glycoprotein from tobacco callus with potential pathogens.

@article{Mellon1982InteractionOA,
  title={Interaction of a hydroxyproline-rich glycoprotein from tobacco callus with potential pathogens.},
  author={Jay E. Mellon and John P. Helgeson},
  journal={Plant physiology},
  year={1982},
  volume={70 2},
  pages={401-5}
}
A hydroxyproline-rich glycoprotein was isolated from tobacco (Nicotiana tabacum L.) callus tissue cultures by an acidic-ethanol extraction procedure and purified to about 95% homogeneity by ion exchange chromatography on carboxymethyl cellulose. This glycoprotein agglutinated cells of an avirulent strain (B-1) of the bacterial pathogen Pseudomonas solanacearum but not its parental, virulent isolate (K-60). Bacterial lipopolysaccharide (from K-60 strain) inhibited this agglutination. The tobacco… CONTINUE READING

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