Interaction of a four-way junction in DNA with T4 endonuclease VII.

@article{Parsons1990InteractionOA,
  title={Interaction of a four-way junction in DNA with T4 endonuclease VII.},
  author={Carol A. Parsons and B{\"o}rries Kemper and Stephen C West},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 16},
  pages={9285-9}
}
The binding of a synthetic four-way junction in DNA by T4 endonuclease VII has been studied using gel retardation and footprint analysis. Two specific protein-DNA complexes have been observed, but only one is stable in the presence of moderate concentrations of salt. The footprint of T4 endonuclease VII in the salt-resistant complex has been probed using hydroxyl radicals generated by the reaction of iron(II)/EDTA with hydrogen peroxide. The hydroxyl radical cleavage pattern indicates… CONTINUE READING

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