Interaction of RNA hairpins with the human U1A N-terminal RNA binding domain.

@article{Hall1994InteractionOR,
  title={Interaction of RNA hairpins with the human U1A N-terminal RNA binding domain.},
  author={Kathleen B Hall},
  journal={Biochemistry},
  year={1994},
  volume={33 33},
  pages={10076-88}
}
The isolated 102 amino acid N-terminal RNA binding domain (RBD) of the human U1A protein specifically interacts with a short RNA hairpin containing the U1 snRNA stem/loop II sequence. This recognition is nucleotide-specific, for substitutions of critical nucleotides in the RNA loop decrease binding affinity up to 10(6)-fold, as measured by nitrocellulose filter binding experiments. The magnitude of the loss of binding free energy with single-nucleotide substitution in the conserved GCA sequence… CONTINUE READING