Interaction of R17 coat protein with synthetic variants of its ribonucleic acid binding site.

@article{Carey1983InteractionOR,
  title={Interaction of R17 coat protein with synthetic variants of its ribonucleic acid binding site.},
  author={Jannette Carey and P. T. Lowary and O. C. Uhlenbeck},
  journal={Biochemistry},
  year={1983},
  volume={22 20},
  pages={4723-30}
}
The specificity of the interaction between R17 coat protein and its site of translational repression on R17 RNA was studied by enzymatically synthesizing 23 sequence variants of the RNA binding site and measuring their affinity to the coat protein by a nitrocellulose filter binding assay. Experiments using oligomers truncated on the 3' and 5' termini allowed precise determination of the edges of the binding domain. Several oligomers which disrupted one or more of the base pairs in the binding… CONTINUE READING

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