Interaction of Intravenous Anesthetics with Recombinant Human M1-M3 Muscarinic Receptors Expressed in Chinese Hamster Ovary Cells

@article{Hirota2002InteractionOI,
  title={Interaction of Intravenous Anesthetics with Recombinant Human M1-M3 Muscarinic Receptors Expressed in Chinese Hamster Ovary Cells},
  author={Kazuyoshi Hirota and Yoshio Hashimoto and David G Lambert},
  journal={Anesthesia \& Analgesia},
  year={2002},
  volume={95},
  pages={1607-1610}
}
Previous reports suggest that the effects of propofol, ketamine, and thiopental on airway tone may be because of modulation of parasympathetic activity. We examined if these anesthetics interact with recombinant human M1-M3 muscarinic receptors expressed in Chinese hamster ovary cells (CHO-M1, M2, and M3) using the displacement of 0.4 nM of l-[N-methyl-3H]scopolamine methyl chloride([3H]NMS). In addition, functional studies were performed by fluorometrically monitoring methacholine (1 mM… Expand
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