Interaction of Escherichia coli heat-stable enterotoxin B with cultured human intestinal epithelial cells.

@article{Chao1997InteractionOE,
  title={Interaction of Escherichia coli heat-stable enterotoxin B with cultured human intestinal epithelial cells.},
  author={K L Chao and Lawrence A. Dreyfus},
  journal={Infection and immunity},
  year={1997},
  volume={65 8},
  pages={3209-17}
}
Binding of Escherichia coli heat-stable enterotoxin B (STb) to the human intestinal epithelial cell lines T84 and HT29 and to polarized T84 cells was studied to define the initial interaction of this peptide toxin with target cells. Equilibrium and competitive binding isotherms showed that 125I-STb bound specifically to T84 and HT29 cells; however, the toxin-epithelial cell interactions could be characterized by low-affinity binding (< or = 10(5) M(-1)) to a high number of binding sites (> or… CONTINUE READING