Interaction of DNA polymerase I (Klenow fragment) with the single-stranded template beyond the site of synthesis.

@article{Turner2003InteractionOD,
  title={Interaction of DNA polymerase I (Klenow fragment) with the single-stranded template beyond the site of synthesis.},
  author={Robert M Turner and Nigel D. F. Grindley and Catherine M. Joyce},
  journal={Biochemistry},
  year={2003},
  volume={42 8},
  pages={2373-85}
}
Cocrystal structures of DNA polymerases from the Pol I (or A) family have provided only limited information about the location of the single-stranded template beyond the site of nucleotide incorporation, revealing contacts with the templating position and its immediate 5' neighbor. No structural information exists for template residues more remote from the polymerase active site. Using a competition binding assay, we have established that Klenow fragment contacts at least the first four… CONTINUE READING

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