Interaction of CD4 with HLA class II antigens and HIV gp120

@article{PiatierTonneau2004InteractionOC,
  title={Interaction of CD4 with HLA class II antigens and HIV gp120},
  author={D. Piatier‐Tonneau and L. Gastinel and F. Amblard and Marianne Wojcik and P. Vaigot and C. Auffray},
  journal={Immunogenetics},
  year={2004},
  volume={34},
  pages={121-128}
}
We have developed a cellular adhesion assay in which B lymphocytes expressing HLA class II antigens form rosettes with COS cells expressing high levels of cell surface CD4 upon transient transfection with a CDM8-CD4 plasmid construct. The assay is specific, quantitative, and overcomes the difficulties encountered with a previously described system using an SV40 viral vector. Rosette formation was inhibited by a series of CD4- and HLA-DR-specific antibodies, as well as by human immunodeficiency… Expand
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