Interaction of 6-phosphofructokinase with cytosolic proteins of Saccharomyces cerevisiae.

@article{Schwock2004InteractionO6,
  title={Interaction of 6-phosphofructokinase with cytosolic proteins of Saccharomyces cerevisiae.},
  author={Joerg Schwock and J{\"u}rgen Kirchberger and Anke Edelmann and Thomas M. Kriegel and Gerhard Kopperschl{\"a}ger},
  journal={Yeast},
  year={2004},
  volume={21 6},
  pages={
          483-94
        }
}
Hetero-octameric 6-phosphofructokinase (Pfk-1) from Saccharomyces cerevisiae is composed of two types of subunits, alpha and beta, which are encoded by the unlinked genes PFK1 and PFK2. Pfk single deletion mutants expressing only one type of subunit exhibit Pfk-1 activity in vivo which, however, is completely lost immediately after cell disruption. In order to elucidate the preconditions of the in vivo activity of the mutant enzymes composed of either alpha- or beta-subunits, we have… CONTINUE READING
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