Interaction between rose bengal and different protein components.

  title={Interaction between rose bengal and different protein components.},
  author={Scheffer C. G. Tseng and Sheng Hua Zhang},
  volume={14 4},
Bindings of rose bengal to several proteins were determined by Sephadex G-75 chromatography. Their respective blocking effect against dye uptake was demonstrated in an assay using a rabbit corneal epithelial cell layer. The total binding capacity of nonmucin proteins was measured using fluorometry and Scatchard analysis. The results showed that albumin, lactoferrin, transferrin, and lysozyme could--but serum prealbumin, IgA, carboxymethyl cellulose (CMC), and Sepharose 4B-purified porcine… CONTINUE READING