Interaction between cystic fibrosis transmembrane conductance regulator and outwardly rectified chloride channels.

@article{Jovov1995InteractionBC,
  title={Interaction between cystic fibrosis transmembrane conductance regulator and outwardly rectified chloride channels.},
  author={Biljana Jovov and Iskander I. Ismailov and Bakhrom K Berdiev and Catherine M. Fuller and Eric Sorscher and John R. Dedman and Marcia A. Kaetzel and Dale J. Benos},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 49},
  pages={29194-200}
}
We have previously described a protocol for the simultaneous isolation and reconstitution of a protein kinase A (PKA)-sensitive outwardly rectified chloride channel (ORCC) and the cystic fibrosis transmembrane conductance regulator (CFTR) from bovine tracheal epithelium. Immunoprecipitation of CFTR from this preparation prevented PKA activation of the ORCC, suggesting that CFTR regulated the ORCC and that this regulatory relationship was preserved throughout the purification procedure. We now… CONTINUE READING