Interaction between antibody-diversification enzyme AID and spliceosome-associated factor CTNNBL1.

@article{Conticello2008InteractionBA,
  title={Interaction between antibody-diversification enzyme AID and spliceosome-associated factor CTNNBL1.},
  author={Silvestro G. Conticello and Karuna Ganesh and Kanmin Xue and Mason Lu and Cristina Rada and Michael Neuberger},
  journal={Molecular cell},
  year={2008},
  volume={31 4},
  pages={474-84}
}
Activation-induced deaminase (AID) deaminates deoxycytidine residues in immunoglobulin genes, triggering antibody diversification. Here, by use of two-hybrid and coimmunoprecipitation assays, we identify CTNNBL1 (also known as NAP) as an AID-specific interactor. Mutants of AID that interfere with CTNNBL1 interaction yield severely diminished hypermutation and class switching. Targeted inactivation of CTNNBL1 in DT40 B cells also considerably diminishes IgV diversification. CTNNBL1 is a widely… CONTINUE READING