Intein Zymogens: Conditional Assembly and Splicing of Split Inteins via Targeted Proteolysis

@inproceedings{Gramespacher2017InteinZC,
  title={Intein
Zymogens: Conditional Assembly and Splicing
of Split Inteins via Targeted Proteolysis},
  author={Josef A. Gramespacher and Adam J. Stevens and Duy Phuong Nguyen and Jason W Chin and Tom W Muir},
  booktitle={Journal of the American Chemical Society},
  year={2017}
}
Naturally split inteins have found widespread use in chemical biology due to their ability to drive the ligation of separately expressed polypeptides through a process termed protein trans-splicing (PTS). In this study, we harness PTS by rendering association of split intein fragments conditional upon the presence of a user-defined protease. We show that these intein "zymogens" can be used to create protein sensors and actuators that respond to the presence of various stimuli, including… CONTINUE READING
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