Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene.

@article{Kurtz1986IntegrativeTO,
  title={Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene.},
  author={Myra B. Kurtz and M W Cortelyou and Donald R. Kirsch},
  journal={Molecular and cellular biology},
  year={1986},
  volume={6 1},
  pages={142-9}
}
Candida albicans is a diploid dimorphic yeast with no known sexual cycle. The development of a DNA transformation system would greatly improve the prospects for genetic analyses of this yeast. Plasmids were isolated from a Candida Sau3A partial library which complements the ade2-1 and ade2-5 mutations in Saccharomyces cerevisiae. These plasmids contain a common region, part of which, when subcloned, produces ade2 complementation. Among the small number of auxotrophs previously isolated in C… CONTINUE READING
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